Mushroom Spawn Bag - A Complete Guide - Mushroom Growing (2024)

This guide will accurately show you how to use a mushroom spawn bag. The guide will provide some more advanced tips to help you best grow mushrooms using a grow bag with many types of mushroom strains and conditions.

Before we move forward, it’s safe to assume you have prepared your mushroom substrate and mushroom spore syringe. If you are new to these concepts, read our guide on mushroom growing at home for a more beginner-focused guide.

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Introduction to the Mushroom Grow Bag

Components of the mushroom spawn bag

  • Each bag has a self-healing injector site, which closes after you pull the needle out.
  • The white square filter patch located towards the top of the bag allows proper gas exchange but will keep contaminates out.


Sterilization tips for mushroom spawn bags

  • It is essential to ensure that all spore injecting activities are done in the cleanest environment possible.
  • Sterilized conditions ensure mold does not form inside your growing substrate, which can ruin your work.


Required Supplies – Mushroom Grow Bags on Amazon

Mushroom Grow Bags

Mushroom Spawn Bag - A Complete Guide - Mushroom Growing (2)Mushroom Spawn Bag - A Complete Guide - Mushroom Growing (3)

Required Supplies – Mushroom Spore Syringes on Amazon

Search Mushroom Spore Syringes on Amazon


Part 1: Inoculating Mushroom Spawn Bag

1. Wipe the syringe needle and the injection site with an alcohol swab and let it dry

2. Pull the syringe needle cover off of the syringe.

3. Push the needle about 1/2 inch into the bag and inject about 1-2 cc’s of spores. Pull the needle halfway out, angle it in a different direction, and inject another 1-2 cc’s of spores. Use a total of 3-5 cc’s of spores per spawn bag.

4. Make sure you inject in multiple locations to help spread your spore solution.

5. Unroll the spawn bag and make sure the filter patch is upright. Slowly pull the two sides apart and be careful not to touch the white filter. The goal is to provide a channel or path from the grain to the filter.

Part 2: Colonization of the Mushroom Spawn Bag

1. Place your spawn bags in a warm, dark location (75-81 degrees) and leave to incubate. Unlike jars, Spawn bags colonize from the inside out, so you most likely won’t see any mycelium growth for 7-14 days.

(Optional) Between 14-17 days, you can break up the bags with your fingers to spread the mycelium. After spreading the white mycelium, gently re-pack the grain to its original shape. This step is optional as you must be careful not to break them up too much and destroy the mycelium network. We only suggest this step when you have very slow-growing bags. This method works best on the Rye bags.

Note: Check out our shop for more mushroom growing supplies.

2. Complete colonization may take 30-45 days, depending on the species and the temperature you incubate. Unlike jars, the speed at that spawn bags germinate and colonize may have much more significant variance. The photo below is a spawn bag full of colonized and ready to move to the next step!

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Part 3: Fruiting Your Spawn Bags

There are three different ways to fruit spawn bags. The most popular and recommended way is the bulk casing method: The yield is four to five times more than the other fruiting methods. Your second-best option for fruiting would be to use a fruiting chamber with perlite like you would substrate jars (See BRF Jars / PF Tek Guide here). The last option is to fruit directly in the bag.

Fruiting using the Bulk Casing Method

This method involves simply breaking up the fully colonized spawn bag into small pieces and mixing it with more mushroom growing substrate in a tub or container. The contents of the mushroom grow bag and new uncolonized substrate re-colonize and form one big fruiting cake.

In order to mix the contents of your spawn bag into more substrate, you will need more of the same substrate you have inside your spawn bag. This means you can buy another spawn bag of the same type, or you can buy a more bulk amount of the same material. This is easy to do if your substrate is a manure mix, straw, or sawdust.

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It will take some time for the substrate from the spawn bag to recolonize the uncolonized substrate, about a week or two. But once it does, the fruiting result will be strong and worth it.

This method provides five times the surface area, resulting in a much larger yield. The bulk Casing Method has become the most popular method for first-time spawn bag growers.

Fruiting in a Humidity Chamber

  • The second most popular way to fruit your spawn bags would be to use a container with dampened perlite (white volcanic rock).
  • In this method, substrate jars are used. You will need vermiculite in this method to provide moisture to the cake and keep it hydrated.
  • Start by removing the colonized grain from the bag. Dunking in water is not recommended with the spawn bags because they will not absorb water directly into the grain. Hence, the need for vermiculite.
  • Place a piece of foil on top of the perlite. The foil should be about one inch larger than the cake on all sides.
  • Place a 1cm layer of vermiculite on the foil. Use a spray bottle and moisten the vermiculite enough, so it’s saturated, but there should be no standing water.
  • Place the spawn cake on the vermiculite and add another 1cm layer of vermiculite. Spray the top layer with water to dampen. You will need to keep the vermiculite damp by misting daily.
  • It can take a few weeks for mushrooms to form. There are typically no set flushes for this growing style; the mushrooms will grow sporadically for the following weeks.

Note: Check out our shop for more mushroom growing supplies.

Fruiting in the Bag

The last method is fruiting directly in the bag. This method is the easiest but typically yields the least. You will need vermiculite for this method to keep the grains hydrated.

  • Cut the top of the bag off. Cut above the white filter patch.
  • Remove the spawn cake. Add a 1cm layer of vermiculite to the bottom of the bag, then use a spray bottle to moisten it enough, so it’s saturated, but you don’t want any standing water. Use spring or bottled water.
  • Put the spawn cake back in the bag and add another 1cm layer of vermiculite on top of the spawn cake. Spray with water like you did the first layer.
  • Fold the top of the bag over and use a binder or paper clip to keep it closed.
  • Open the bag twice daily for a few minutes to allow fresh air to exchange. Mist the vermiculite as needed to ensure it stays hydrated.
  • It can take a few weeks for mushrooms to form. There are typically no set flushes for this growing style; the mushrooms will grow sporadically for the following weeks.

Congratulations, you’ve completed the steps to use a mushroom grow spawn bag!

Visit our main page for more guides and tips!: https://mushroomgrowing.org.

Mushroom Spawn Bag - A Complete Guide - Mushroom Growing (2024)

FAQs

Do mushroom grow bags work? ›

Yes, you can grow mushrooms indoors using a grow bag. Grow bags provide a suitable environment for mushroom cultivation by maintaining ideal humidity levels and allowing proper air circulation. They're designed with a sterile substrate that's enriched with essential nutrients for the mushrooms to thrive.

How long do all in one mushroom bags take to grow? ›

Storage & Colonization

Keeping your bag at the higher end of the temperature range will encourage faster colonization. 2. After about 4-8 weeks, you should start to see mycelium growing on the substrate. You'll notice the white mycelium growing outward from the point of injection.

Where is the best place to put a mushroom grow bag? ›

While this can be good, airflow is also important and small enclosed spaces can cause your mushrooms to suffer from lack of oxygen. We recommend placing your grow kit on your kitchen bench, out of direct sunlight.

How much does a mushroom bag yield? ›

While some growers report harvests of 10-15 grams of dried mushrooms per pound of substrate in these bags, others might achieve less, often from significant side pinning, and especially in the case of contamination or suboptimal growing conditions.

What are the disadvantages of grow bags? ›

What are the Disadvantages of Using Grow Bags? They can dry out quickly, especially in hot weather, requiring more frequent watering. They may not be suitable for plants that require deep soil. Growbags can be easily punctured or torn, which can cause soil to spill out.

Are grow bags worth it? ›

They are a good choice if you are growing a whole bed of plants. In between, there are grow bag sizes for many other plant options. Grow bags can be a great choice to grow plants like lettuce, potatoes, eggplants, flowers, and leafy greens.

Do mushroom bags need air? ›

The top of the bag creates a humid dome for the mushrooms to fruit. Keep in mind that there won't be a lot of fresh air inside the bag, so if you are using the bag to fruit your mushrooms, ensure that you open the top of the bag often in order to get adequate fresh air exchange.

How can I speed up mycelium growth? ›

The higher the inoculation rate or amount of spawn added to the substrate, the faster the mycelium will grow through the substrate. The substrate likely will not produce a larger amount of mushrooms, so a high inoculation rate means more money spent on spawn per pound of mushrooms.

Do mushroom grow kits keep producing? ›

Mushroom kits are not reusable, but most of them produce at least 2 harvests' worth of mushrooms.

Do you water mushroom grow bags? ›

You don't need to water mushrooms like a plant.

The spray bottle is for creating humidity. Humidity is a key factor for successful mushroom growth. Spray the area around your kit with a few pumps of water whenever you have a chance, at least 2-3 times a day.

Do mushroom bags need light? ›

⇒ Typically most people have always colonized spawn bags in the dark like the jars. Recent testing and research has shown that light doesn't have any effect on colonizing. Since premature pinning before 100% colonization doesn't happen with spawn bags, do whatever is convenient for your setup.

Why is my mushroom bag not growing? ›

Another reason is that even viable spores may not have the right conditions to germinate, such as the right temperature, humidity, or availability of nutrients. Additionally, many spores may be competing with one another for the same limited resources, and only a few may be able to survive and grow into mushrooms.

Is a mushroom business worth it? ›

Mushroom cultivation can be profitable due to its low startup costs and the ability to produce a high yield in a small amount of space. Mushrooms also have a short time it takes to grow from spores to full size, with some types taking as little as 30 days.

What is the best substrate for mushroom grow bags? ›

A successful mushroom substrate must have the right nutrient content, moisture levels, pH balance, and be properly sterilized to support mycelium growth. Combinations of straw and sawdust, compost and manure, coffee grounds and sawdust, straw and coffee grounds, and compost and sawdust are optimal for high yields.

Is my mushroom bag ready to fruit? ›

After a few days, you will start to see little white spots forming in your substrate. Eventually, these will take over the entire bag. For oyster mushrooms, the entire process takes about two to three weeks. You will know your bag is ready to fruit when the entire substrate is covered with bright white mycelium.

Are grow bags better than compost? ›

If you are making your own grow bags it is important to note that grow bag compost may tend to hold water better and have more nutrients added as compared to a general multi-purpose compost.

How long do mycelium bags last? ›

The dehydrated GIY material is shelf stable and should be used within 10 weeks of the date on the bag's label, but lasts longer if refrigerated. Living GIY material must be kept refrigerated at all times and used within 3 months.

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